Metabolism and transcriptional regulation in chilling injury development of nectarine fruit during postharvest cold storage

Abstract

Peach fruit is susceptible to chilling injury during postharvest long-term cold storage, but the understanding of metabolic and transcriptional reaction of nectarine cultivar fruit to cold storage remains incomplete. In this study, nectarine fruit was used for a low temperature treatment, which gradually leading to the deterioration of fruit quality. Comparative metabolome and transcriptome analyses were performed to comprehensively understand the metabolic and transcriptional changes occurring during cold storage in nectarine fruit. The result showed that numerous primary and secondary metabolites exhibited increased levels following the low-temperature treatment. And there were 3400 differentially expressed genes continuously regulated by cold storage, which were mostly enriched in metabolic and secondary metabolite biosynthesis pathways. The combined pathways enrichment of differential metabolites and genes were primarily centered on secondary metabolite biosynthesis. Correspondingly, a strong correlation was observed between changes in secondary metabolites and the expression of key enzymes including 4-coumarate CoA ligase (4CL), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), flavonol synthase (FLS), dihydroflavonol reductase (DFR), and UDP-glycose flavonoid glycosyltransferase (UFGT), which were involved in flavonoids biosynthesis. Additionally, several fruit quality characteristics (carotenoids, cell wall components, antioxidant enzymes) and stress related signaling genes were markedly affected by cold storage. These findings offer new insights into the networks governing metabolic and transcriptional regulation during nectarine fruit cold storage.