Metabolic signature of electrosurgical liver dissection

Witigo Von Schönfels ,T Becker ,J Hampe ,M Ahrens ,B Sipos ,P Huebbe ,F Stickel ,C Röcken ,B Schniewind ,O Von Kampen ,K Richter ,O Will ,J Egberts ,Sebastian Hinz ,M Nothnagel ,G Rimbach ,E Patsenker ,K Balschun ,S Schreiber ,A Landrock ,C Schafmayer

doi:10.1055/s-0032-1332037

Abstract

Background and Aims: High frequency electrosurgery has a key role in the broadening application of liver surgery. Its molecular signature, i.e. the metabolites evolving from electrocauterization which may inhibit hepatic wound healing, have not been systematically studied.

Highlights

The indications and use of liver surgery have increased substantially over the last decade
Between two and three independent paired liver samples were obtained from six individuals, extracted and subjected to metabolic screening using gas chromatography/mass spectrometry (GC/mass spectrometry (MS))
As an exploratory step to evaluate the global metabolite pattern with regards to the effects of ES, a principal components analysis was performed in the discovery sample set

Summary

Introduction

The indications and use of liver surgery have increased substantially over the last decade. ES preparation induces significant thermic and oxidative damage to the liver. This surgical trauma is associated with a typical decline in liver function during the immediate postoperative period and clearly affects patient survival [6,7] resulting from a loss of liver parenchymal reserve in addition to thermal injury to the remaining parenchyma. Measures to minimize electro surgery-derived tissue trauma could potentially improve postoperative outcomes. To develop such interventions, a better knowledge of the toxic metabolites evolving from ES, and their molecular effects on liver cell regeneration is required. I.e. the metabolites evolving from electrocauterization which may inhibit hepatic wound healing, have not been systematically studied

Methods

Results

Conclusion