Metabolic responses of brown planthoppers to IR56 resistant rice cultivar containing multiple resistance genes

Abstract

The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive pests in rice production, and rice resistance is thought to be an economical and environmentally friendly strategy against BPH. Although resistant rice cultivars have been widely applied to control BPH, little is known regarding the impact of the ingestion of resistant plant phloem on the BPH physiological metabolism. In this study, the differences in the metabolic responses of BPH nymphs during the first 72 h after ingesting susceptible TN1 and resistant IR56 plant phloem were compared. The results showed that BPH nymphs feeding on IR56 plants exhibited significant decreases in honeydew excretion and body weight, and significantly lower concentrations of most of the detected sugars, vitamins and some essential amino acids but higher levels of most amides, free fatty acids and some non-essential amino acids. These findings indicate that the energy metabolism and nutrition supply of these nymphs were disturbed by the resistant rice plants. The qPCR results revealed that BPH could actively adapt to IR56 plants by upregulating the gene expression levels of some detoxification enzymes, including GST, CarE and POD, to some extent. These results provide additional information to improve our understanding of physiological mechanism underlying the loss of BPH fitness caused by resistant rice varieties.