Metabolic responses and microbial community changes to long chain fatty acids: Ammonia synergetic co-inhibition effect during biomethanation

Abstract

Anaerobic co-digestion is an established strategy for increasing methane production of substrates. However, substrates rich in proteins and lipids could cause a long chain fatty acids (LCFA)-ammonia synergetic co-inhibition effect. The microbial mechanisms of this co-inhibition are still unclear. The current study explored the effect of the synergetic co-inhibition on microbial community changes and prediction of metabolic enzymes to reveal the microbial mechanisms of the co-inhibition effect. The results indicated that during the synergetic co-inhibition, methanogens were mainly affected by ammonia. Decreased relative abundances of Petrimonas (82%) and Paraclostridium (67%) showed that ammonia inhibition contributed to the suppression of LCFA β-oxidation under the synergetic co-inhibition conditions. The accumulation of more LCFA could further suppress microorganisms’ activities involved in several steps of anaerobic digestion. Finally, decrease of critical enzymes’ abundances confirmed the synergetic co-inhibition effect. Overall, the current study provides novel insights for the alleviation of synergetic co-inhibition during anaerobic digestion.