Abstract
Heat stress seriously threatens the growth of Pleurotus ostreatus. Various studies have been performed to study the resistance of P. ostreatus to heat stress. Here, the metabolome was evaluated to determine the response of P. ostreatus mycelia to heat stress at different times (6, 12, 24, 48 h). More than 70 differential metabolites were detected and enriched in their metabolic pathways. Dynamic metabolites changes in enrichment pathways under heat stress showed that heat stress enhanced the degradation of unsaturated fatty acids and nucleotides, increased the content of amino acids and vitamins, and accelerated glycolysis and the tricarboxylic acid cycle in P. ostreatus. The time course changes of P. ostreatus metabolites under continuous heat stress demonstrated that amino acids continuously changed with heat stress, nucleotides clearly changed with heat stress at 12 and 48 h, and lipids exhibited an increasing trend with prolonged heat stress, while few types saccharides and vitamins changed under heat stress. Additionally, heat-treated P. ostreatus produced salicylic acid and other stress-resistant substances that were reported in plants. This study first reported the metabolites changes in P. ostreatus mycelia during 48 h of heat stress. The metabolic pathways and substances that changed with heat stress in this research will aid future studies on the resistance of P. ostreatus and other edible fungi to heat stress.
Highlights
Pleurotus ostreatus has economic and ecological values and medicinal properties and has been intensely studied and cultivated in many different areas of the world (Sánchez, 2010)
We first identified the dynamic alteration of P. ostreatus mycelia metabolites during 48 h of heat stress, FIGURE 10 | Metabolic network of P. ostreatus under heat treatment
Thereby determining the intracellular metabolic changes that occurred in P. ostreatus mycelia in response to heat stress, which mainly included enhanced unsaturated fatty acid and nucleotide degradation, increased content of amino acid and vitamin and increased synthesis of some stress-resistant substances
Summary
Pleurotus ostreatus has economic and ecological values and medicinal properties and has been intensely studied and cultivated in many different areas of the world (Sánchez, 2010). High temperature is the most adverse stress in the cultivation of P. ostreatus. After high-temperature stress, mycelial growth is inhibited, mycelia are susceptible to infection by pathogens, such as Trichoderma asperellum, and apoptotic-like cell death can occur (Song et al, 2014; Qiu et al, 2017). These adverse effects cause enormous economic losses, which urgently call for studies on the mechanism of P. ostreatus resistance to heat stress. Cell signals were involved in the heat stress response. Kong et al (2012) discovered that nitric oxide
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