Abstract
1. 1. Cold-stored red blood cells of guinea pig lose K + more rapidly with Ca 2+ in the medium than without. They also lose K + more rapidly if deprived of glucose. The two effects are independent and do not involve the Na/K pump. 2. 2. In cold-stored glucose-deprived cells loss of K + parallels decrease in reduced glutathione (GSH) concentration. 3. 3. Lowering GSH in fresh cells reversibly increases passive K + permeation. Thus, GSH is a likely metabolic regulator of passive permeability.
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