Abstract
Effect of the specific gene knockout on the main metabolism in Escherichia coli was reviewed, and the regulation mechanisms were clarified based on different levels of information such as gene expressions, enzyme activities, intracellular metabolite concentrations, and metabolic fluxes together with fermentation data. The effects of the knockout of such genes as pflA, pta, ppc, pykF, adhE, and ldhA on the metabolic changes were analyzed for the case under anaerobic condition. The effects of the knockout of such genes as pgi, zwf, gnd, ppc pck, pyk, and lpdA on the metabolic changes were also analyzed for the case under aerobic condition. The metabolic regulation analysis was made focusing on the roles of transcription factors.
Highlights
Escherichia coli is a Gram-negative, facultative anaerobic and non-sporulating bacterium
Effect of the specific gene knockout on the main metabolism in Escherichia coli was reviewed, and the regulation mechanisms were clarified based on different levels of information such as gene expressions, enzyme activities, intracellular metabolite concentrations, and metabolic fluxes together with fermentation data
REGULATION OF THE METABOLISM BETWEEN ANAEROBIC AND AEROBIC CONDITIONS. Global regulators such as Fnr and ArcAB are mainly responsible for the regulation of the metabolism under oxygen limited condition, where Fnr regulates the expressions of metabolic pathway genes under anaerobic
Summary
Escherichia coli is a Gram-negative, facultative anaerobic and non-sporulating bacterium. The E. coli cells are typically rod-shaped with length of about 1 - 2 μm having a cell volume of 0.6 - 0.7 μm3 This organism has been identified by Theodor Escherich in 1885, and is classified as part of Enterobacteriaceae family of gamma-proteobacteria [1]. This bacterium grows optimally at 37 ̊C and can survive even at higher temperature up to about 50 ̊C. An attempt is made to clarify the regulation mechanisms for the several specific gene knockout mutants under both anaerobic and aerobic conditions based on different levels of information focusing on the roles of transcription factors
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