Metabolic Reconstruction of Glutamate-Glutamine Cycling: A Flux Balance Approach

Abstract

Event Abstract Back to Event Metabolic Reconstruction of Glutamate-Glutamine Cycling: A Flux Balance Approach Nelson E. Vega-Vela1, Cynthia M. Jimenez1, George E. Barreto1* and Janneth Gonzalez1 1 Pontificia Universidad Javeriana, Colombia Outline - Astrocyte/Neuron metabolic relationships: A short overview - Metabolic reconstruction: Basic principles - Computational systems biology databases and toolkits: Short review - Metabolic modelling and flux balance analysis of glutamate-glutamine cycle between neurons and astrocytes - Metabolic network analysis and visualization Abstract Cells, tissues, organs and organisms can be understood as a large number of interconnected networks of biochemical pathways, genes, transcripts and proteins which give rise to emergent and specific functions and behaviors in a complex biological system. The complexity exhibited by these systems imposes considerable challenges to understand how they behave and work. For instance, diseases or patho-physiological states are complex conditions that must be considered in a holistic approach because they involve intricate interactions between thousands of components such as genes, transcripts and proteins. In this context, diseases are considered as perturbed states of these networks which can be used to identify components (e.g. metabolites, enzymes, etc.) and interactions that are gained or lost, as well as the biochemical pathways involved. Therefore, discrimination of components and interactions that are not relevant or informative is important to embrace the complexity for understanding biological processes. Systems biology, Network medicine and the recent Systems neuroscience has been successfully applied to make this complexity comprehensible in the different fields of biology, physiology, medicine and neuroscience. To illustrate these approaches within the framework of the workshop “Latin-American School on glial cells in the diseased brain”, we reconstruct the well-known glutamate/glutamine cycle to understand how glutamate can be a neurotoxic agent through glutamate-induced excitotoxicity in neurons and astrocytes. Figure 1 References Barreto GE, Santos-Galindo M, Garcia-Segura LM. Selective estrogen receptor modulators regulate reactive microglia after penetrating brain injury. Front Aging Neurosci, 6:132, 2014. Cabezas R, Ávila M, Gonzalez J, El-Bachá RS, Báez E, Garcia-Segura LM, Jurado Coronel JC, Capani F, Cardona-Gomez GP, Barreto GE. Astrocytic modulation of blood brain barrier: perspectives on Parkinson’s disease. Front. Cell. Neurosci. 8:211, 2014. Barreto GE, White RE, Xu L, Palm CJ, Giffard RG. Effects of heat shock protein 72 (Hsp72) on evolution of astrocyte activation following stroke in the mouse. Exp Neurol. 238(2):284-296, 2012. Barreto G , White RE, Ouyang Y, Xu L, Giffard RG. Astrocytes: targets for neuroprotection in stroke. Cent Nerv Syst Agents Med Chem, 11(2):164-73, 2011. Barreto GE , González J, Torres Y, Morales L. Astrocyte-neuronal crosstalk: Implications for neuroprotection from brain injury. Neurosc Res, 71(2):107-13, 2011. Keywords: astrocyte, Neurons, Metabolic Networks, Flux balance analysis, Neurotoxicity, Computational Biology Conference: Latin-American School on glial cells in the diseased brain (IBRO), Bogotá, Colombia, 13 Jul - 17 Jul, 2015. Presentation Type: Oral Presentation Topic: Neural excitability, synaptic transmission, glia: Cellular and molecular mechanisms Citation: Vega-Vela N, Jimenez CM, Barreto G and Gonzalez J (2015). Metabolic Reconstruction of Glutamate-Glutamine Cycling: A Flux Balance Approach. Conference Abstract: Latin-American School on glial cells in the diseased brain (IBRO). doi: 10.3389/conf.fncel.2015.35.00008 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Feb 2015; Published Online: 16 May 2015. * Correspondence: Prof. George E. Barreto, Pontificia Universidad Javeriana, Bogotá, Colombia, gesbarreto@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nelson E. Vega-Vela Cynthia M Jimenez George E. Barreto Janneth Gonzalez Google Nelson E. Vega-Vela Cynthia M Jimenez George E. Barreto Janneth Gonzalez Google Scholar Nelson E. Vega-Vela Cynthia M Jimenez George E. Barreto Janneth Gonzalez PubMed Nelson E. Vega-Vela Cynthia M Jimenez George E. Barreto Janneth Gonzalez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.