Abstract

This study aims at developing a tissue-specific model for glycolysis in bovine mammary gland epithelial cells by incorporating gene expression data into metabolic reactions. Two types of data sets were embedded in the COnstraint-Based Reconstruction Analysis (COBRA) toolbox: metabolic reactions that overlay bovine genetic information into human glycolysis data from a public database and gene expression data of cattle acquired from lab experiments. As a result, we successfully generated a tissue-specific model of bovine glycolysis in bovine mammary gland epithelial cells, providing information on expression of metabolic pathways and gene annotations still required for curation.

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