METABOLIC PROFILE OF BIFIDOFLORA UNDER DIFFERENT MICROECO-LOGICAL CONDITIONS OF THE COLON BIOTOPE IN HUMAN

Abstract

To study the spectrum and level of short-chained fatty acids (SCFA) in supernatant of bifidobacteria under different microecological conditions ofthe colon biotope in human. Metabolites of 88 bifidobacteria strains isolated from patients when examined for dysbiosis of the colon were investigated. Definition of concentration of SCFA was performed on acidified supernatant samples by a separation method on chromatograph GC-2010 Plus, Shimadzu (Japan). Monobasic acids were found in metabolites of 50 - 100% study cultures of bifidobacteria where the spectrum and level of carboxylic acids in supernatants varied depen- ding on microecological condition of the origin of the discharge. In severe damages of microsymbiocenosis in metabolites of Bifidobacterium spp., summarized concentrations of SCFA, structural index, levels of aceitic and propionic acids were decreased. Strain-specific differences in a metabolic profile of bifidofloia in a composition of individual consortiums were determined. Data obtained indicate the variation of functional (metabolic) activity of dominant strains in different microecological conditions of the human colon. Uniquieness of metabolome of every other strain due to their strain specifity determines their functional activity, but a metabolic profile of bifidoflora can serve as the most important criterion for the selection of effective probi- otic drugs for treatment and prevention of dysbioisis in the colon.