Abstract
We investigated the metabolic outcome of different coping strategies in 129S6/SvEvTac (129Sv) and C57BL/6Ntac (Bl6) strains. Two different batches of male 129Sv and Bl6 mice were used. One batch was not subjected to any behavioral manipulations (home cage control; HCC), whereas the other batch was treated with saline for 11 days and exposed after every treatment to the motor activity measurement (repeated motility tested; RMT). Bl6 RMT mice displayed a robust increase in number of rearings during repeated testing. 129Sv RMT mice experienced significant loss of body weight, but showed enhanced weight gain in HCC batch compared to Bl6. Serum metabolites (acylcarnitines, amino acids, biogenic amines, hexoses, glycerophospholipids and sphingolipids) were determined with AbsoluteIDQ p180 kit. Results of the metabolomic study revealed prominent peculiarities between strains in two different conditions. Comparison of both batches of mice demonstrated that in Bl6 biogenic amines (acetyl-ornithine, alpha-amionadipic acid, carnosine) and lysophosphatidylcholine PC(16:1/0:0) dominated. However in 129Sv acylcarnitine C5 clearly dominated, indicating shift towards short-chain acylcarnitines. Stable strain-specific ratios also emerged for both lines, ratio of glycine/PC ae C38:2 for Bl6 and ratios of C5/C0 as well as PC(16:0/0:0)/PC(16:1/0:0) for 129Sv. The described metabolic changes probably reflect different behavioral coping strategies of 129Sv and Bl6 mice.
Highlights
Bl6 and 129Sv are widely used mouse lines in biomedical research
After Bonferroni correction 13 metabolites were significantly different in comparison of 129Sv and Bl6 mouse lines; more precisely 5 metabolites showed higher values in Bl6 mice (Table 1) and 8 metabolites were elevated in 129Sv (Table 2)
The 129Sv responded differently in RMT compared to home cage control (HCC), with a significant reduction of body weight, a change not seen in Bl6
Summary
Bl6 and 129Sv are widely used mouse lines in biomedical research. The classical method of creating transgenic mice is based on these two lines: 129Sv derived mouse embryonic stem cells are applied for introducing targeted mutations into mouse genome[1], and the Bl6 strain is employed as a background line in transgenic studies[2]. Environmental enrichment seems to reinforce existing predispositions in both strains by inducing an active coping strategy in Bl6 and passive coping strategy in 129Sv6 These strain differences in the exploratory drive and motivational system of www.nature.com/scientificreports/. In HCC 24 acylcarnitines, 35 amino acids and biogenic amines, level of hexoses, 89 glycerophospholipids (13 lysophosphatidylcholine acyls, 38 phosphatidylcholine diacyls, 38 phosphatidylcholine acyl-alkyls), and 15 sphingolipids. In RMT 24 acylcarnitines, 36 amino acids and biogenic amines, level of hexoses, 86 glycerophospholipids (13 lysophosphatidylcholine acyls, 37 phosphatidylcholine diacyls, 36 phosphatidylcholine acyl-alkyls), and 13 sphingolipids. Such a comparative study has not been performed in these mouse lines
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