Abstract
Bacteria can use n-hexadecane as a carbon source, but it remains incompletely understood whether n-hexadecane is transformed into metabolic intermediates prior to cellular uptake or not. We newly isolated a strain identified as Pseudomonas synxantha LSH-7′ and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. Furthermore, we described the identification of extracellular alkane hydroxylase and alcohol dehydrogenase activity; acidification of the culture medium; identification of hexadecanoic acid in the culture medium by the GC-MS analysis; and variation concentration of intracellular n-hexadecane and hexadecanoic acid. A detailed analysis of the experimental data revealed the chemotaxis of this bacterial strain towards n-hexadecane instead of its metabolic intermediates. Our results further suggested that only a fraction of total n-hexadecane followed this path, and alkane hydrolase and hexadecanol dehydrogenase were constitutively expressed when grown in the medium of n-hexadecane. Most strikingly, we quantitatively investigated the concentration of n-hexadecane adsorbed by bacterial chemotaxis. Our findings provided an original insight n-hexadecane might be converted to hexadecanoic acid extracellularly before it was taken up across the cell membrane.
Highlights
Singer and Finnerty[4] observed terminal n-hexadecane degradation by Acinetobacter HO1-N and Pseudomonas putida, and identified five intermediates as n-hexadecylhydroperoxide, n-hexadecanol, n-hexadecyldehyde, n-hexadecanoic acid and n-hexadecylhexadecanoate in degradation process
This work was to report the concentration of n-hexadecane was absorbed quantitatively in the aspect of bacterial chemotaxis and revealed the chemotaxis of this bacterial strain moved towards n-hexadecane instead of its metabolic intermediates
The strains selected to structure the phylogenetic trees had above 99% of similarity with Pseudomonas genus on the nucleotide sequence
Summary
Pseudomonas synxantha LSH-7′: from chemotaxis to uptake of received: 10 May 2016 accepted: 17 November 2016. We newly isolated a strain identified as Pseudomonas synxantha LSH-7′ and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. A detailed analysis of the experimental data revealed the chemotaxis of this bacterial strain towards n-hexadecane instead of its metabolic intermediates. The underlying mechanisms that control the early responses of bacterial cells to n-hexadecane, such as chemotaxis and uptake, remain largely unexplored. Even if the mechanisms that long-chain fatty acids entered cells had been understood[16], little is known about whether n-hexadecane is converted to metabolic intermediates extracellularly before it is taken up across the cell membrane or not. Our investigation were to explore metabolic pathway of n-hexadecane for newly isolated bacterial strain from chemotaxis to uptake. To know whether n-hexadecane was transformed into metabolic intermediates prior to cellular uptake or not
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
