Metabolic inductions in the rat after an intraperitoneal injection of fructose and glucose, according to the nature of the dietary carbohydrate. II. Modifications after seven months of diet.

Abstract

Wistar CF male rats, 120-125 g body wt., were fed during 7 months a diet containing either 72% fructose, or 72% glucose, or 72% starch. They were killed after a 18-hour fast, and either at 0 or at 30 min after an i.p. injection of 2.5 g fructose + 2.5 g glucose/kg body wt. A number of substrates and cofactors were enzymatically assayed in their liver and also in their heart, to study their metabolic state. The fructose fed rats had the worst growth, but the heaviest liver, with a high nitrogen contents. Their cholesterol blood level was very significantly higher than that of the 2 other groups. The profile of the liver concentrations after fasting and their response after the rats had received fructose + glucose, showed that the fructose-fed animals were the best adapted to rapid metabolizing of the carbohydrate: they had the largest increase in a-glycerophosphate and malate and the lowest decrease in total adenine mononucleotide concentration.