Abstract
We characterized the metabolites of 1-methyl-1, 2,3,4-tetrahydro-β-carboline (MTBC) in human urine by gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) and developed an analytical method using GC-NICIMS for their quantitative determination. When tetradeuterated MTBC was orally administered to a human subject, two peaks of the deuterated metabolites appeared on mass fragmentograms of the urine samples after administration. They were identified as tetradeuterated 6-hydroxy-MTBC (6-OH-MTBC) and 7-hydroxy-MTBC (7-OH-MTBC), indicating that MTBC was metabolically hydroxylated in humans. The proposed GC-NICIMS method could sensitively and selectively determine urinary 6-OH-MTBC and 7-OH-MTBC without interference from their artifactual formation during analysis. Its application to urine analysis has revealed that MTBC is excreted in human urine predominantly as the two hydroxylated metabolites, in which 6-OH-MTBC is present in both free and conjugated forms, whereas the 7-OH-MTBC of a conjugated form is much more than the 7-OH-MTBC of a free form.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
