Metabolic, follicular and embryo production responses of postpartum crossbred Holstein × Gir dairy cows fed diets with different energy levels

Abstract

The objective of the present study was to evaluate the effect of two diets with different energy levels and two genetic groups (3/4 and 7/8 Holstein × Gir (HG) cows) on the metabolic and hormonal changes and on the production profile of oocytes and embryos in dairy cows during the early postpartum period. The concentrations of oestrogen, progesterone, glucose, insulin, insulin-like growth factor, urea and non-esterified fatty acids in follicular fluid, as well as blood plasma concentrations of glucose, insulin, urea and non-esterified fatty acids, were evaluated. Oocyte collection was performed every 14 days after parturition. After classification, the oocytes were submitted to in vitro embryo production and cleavage, and blastocyst rates were evaluated. Five days after oocyte collection, the dominant follicle was measured and punctured for follicular fluid retrieval. The high-energy diet increased plasmatic insulin and glucose. The 3/4 HG cows presented a higher plasmatic concentration of insulin, glucose and urea. The hormonal and metabolic changes in plasma were not observed in the follicular fluid. The follicular fluid concentration of IGF-I was increased in cows fed the high-energy diet as well as in the 3/4 HG cows. A higher number of total and viable oocytes was recovered in the 3/4 HG cows, but the 7/8 HG cows had a higher cleavage rate. In conclusion, the high-energy diet was more efficient in maintaining the energy status of crossbred cows, as evidenced by their plasma metabolites and follicular fluid, and 3/4 HG cows were more efficient than 7/8 HG cows at producing oocytes in the early postpartum period.