Abstract
BackgroundThe radix of Angelica sinensis is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary.ResultsPrincipal component analysis of the UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of Angelica sinensis is closely related to the growth periods.ConclusionsThe UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of Angelica sinensis during growth.
Highlights
The radix of Angelica sinensis is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary
We describe a high-throughput and reliable ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-Time-of-flight mass spectrometry (TOFMS)) based analytical method coupled with Principal component analysis (PCA) to profile samples of Angelica sinensis during growth
Identification of components in Angelica sinensis In order to identify components in Angelica sinensis, qualitative analysis of compounds in Angelica sinensis were carried out on an UPLC coupled to electrospray ionization interface (ESI) quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) in ESI+ and ESI- modes
Summary
Principal component analysis of the UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of Angelica sinensis is closely related to the growth periods
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
