Abstract
The current knowledge on the development of recombinant Escherichia coli for the production of polyhydroxyalkanoates (PHAs) is provided. Recombinant E. coli transformed with a stable high copy number plasmid containing the Ralstonia eutropha PHA biosynthesis genes accumulated large amount of poly(3-hydroxybutyrate) [P(3HB)], and P(3HB) production was enhanced by suppressing cell filamentation during P(3HB) accumulation. Other PHAs were synthesized by recombinant E. coli. Several different recombinant E. coli strains were developed to produce PHAs from cheap substrates. The molecular weight of P(3HB) was controlled in recombinant E. coli.
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