Abstract
BackgroundButane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.ResultsThe production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet–PT7–budB–PT7–budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.ConclusionsThe engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-015-0324-x) contains supplementary material, which is available to authorized users.
Highlights
Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD
It can be used as a starting material for the synthesis of bulk chemicals including methylethylketone, gamma-butyrolactone and 1,3-butadiene [1,2,3]
Two molecules of pyruvate are condensed to α-acetolactate by acetolactate synthase (ALS)
Summary
Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production. Butane-2,3-diol (2,3-BD) is a promising commodity biochemical that can be produced by biotechnological routes. It can be used as a starting material for the synthesis of bulk chemicals including methylethylketone, gamma-butyrolactone and 1,3-butadiene [1,2,3]. The heating value of 2,3-BD (27,200 J/g) is comparable with that of other liquid fuels, e.g., methanol (22,100 J/g) and ethanol (29,100 J/g). There are three isomeric forms of 2,3-BD: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD.
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