Abstract
Organophosphate compounds are widely used in pesticides to control weeds, crop diseases, and insect pests. Unfortunately, these synthetic compounds are hazardous and toxic to all types of living organisms. In the present work, Escherichia coli was bioengineered to achieve methyl parathion (MP) degradation via the introduction of six synthetic genes, namely, opdS, pnpAS, pnpBS, pnpCS, pnpDS, and pnpES, to obtain a new transformant, BL-MP. MP and its subsequent decomposition intermediates were completely degraded by this transformant to enter the metabolites of multiple anabolic pathways. The MP-degraded strain created in this study may be a promising candidate for the bioremediation of MP and potential toxic intermediates.
Highlights
Methyl parathion (MP; O,O-dimethyl O-4 nitrophenyl phosphorothioate) is an organophosphorus pesticide that is widely used in agriculture to protect agricultural crops from insect pests and, increase food production (Bhatt et al, 2021b)
Complete degradation of methyl parathion (MP) was achieved within 2 h (Figure 4A). These results demonstrate that BL-MP has a strong MP-degrading ability
This work confirms that organophosphate bioremediation may be achieved by using engineered microorganisms
Summary
Methyl parathion (MP; O,O-dimethyl O-4 nitrophenyl phosphorothioate) is an organophosphorus pesticide that is widely used in agriculture to protect agricultural crops from insect pests and, increase food production (Bhatt et al, 2021b). MP could be slowly hydrolyzed (t1/2 = 68 days at pH 5, t1/2 = 40 days at pH 7, and t1/2 = 33 days at pH 9) in buffer solutions and slowly photodegraded (t1/2 = 61 days) on soil surfaces (US Environmental Protection Agency [USEPA], 1999). The high environmental persistence of MP residues in food and water could impose great threats (Huang et al, 2010). Delayed neurotoxicity and polyneuropathy have been described as the effects of MP toxicity and its residual concentration (Hsieh et al, 2001). MP has been classified as extremely hazardous and listed in the HazDat database of “Chemicals Detected in Surface and/or Groundwater” at the National Priorities List (NPL) sites (WHO, 2004)
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