Abstract

Bacteria produces metabolites and their metabolic pathway can be manipulated to produce good food ingredients. These ingredients includes different amino acids, vitamins, carbohydrates, organic acids aroma compounds and bacteriocins. Different genes in different strains of bacteria are identified and harnessed to attain food ingredients and results of manipulation were satisfying. The metabolic pathway of lactic acid bacteria is manipulated to produce amino acids such as L-alanine was produced by silencing the gene that encodes alanine racemase. Moreover, low calorie sugars such as mannitol and tagatose were produced. Lac-lactis is engineered by silencing lacC and lacD genes for the production of tagatose.

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