Abstract
1. The activities of enzymes involved in glucose utilization via the Embden-Meyerhof and pentose phosphate pathways and the Krebs cycle were studied in fractions prepared from homogenates of intact sheep thyroid glands and isolated cells liberated from follicles by a continuous-flow trypsinization procedure. 2. The specific activities (m∧moles of substrate converted/min/mg protein) of all soluble (nonparticulate) enzymes studied were about 3 times higher in homogenate fractions obtained from cells than in those obtained from intact follicles. This attests to the usefulness of the isolated cell preparation for the study of metabolism in the thyroid gland. 3. Glucokinase activity was found both in mitochondria and in the high-speed supernatant fraction (100,000 ×g). About 70% of the glucokinase activity was associated with mitochondria isolated from both the intact sheep thyroid tissue and the liberated parenchymal cells. 4. No evidence was found for the existence in either mitochondria or cell sap of a glu...
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