Abstract
The effect of oligochitosan O-80 treatment on metabolic changes in white radish (Raphanus sativus L.) sprouts (WRS) was investigated for its potential to enhance bioactive compounds accumulation. The seeds were germinated in deionized water containing different concentrations of oligochitosan O-80 (0 (control), 5, 10, 20 and 40 ppm). Seven-day old sprouts were harvested for metabolome analysis using liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC) for phenolic compounds and glucosinolate analysis, respectively, and spectrophotometric assays to determine the total phenolic content and antioxidant capacity. Partial least squares discriminant analysis was adopted to model the data from the LC-MS and revealed that O-80 at all tested concentrations affected the metabolite profiles of the treated WRS samples. The UV chromatogram at 320 nm showed increased levels of most sinapoyl derivatives, consistent with the increased total phenolic contents. Interestingly, glucoraphasatin (4-methylthio-3-butenyl glucosinolate), a major glucosinolate detected by HPLC, was increased by 40% in the sprouts treated with 10 ppm O-80. Our results provide compelling evidence regarding the exogenous application of oligochitosan O-80 as an elicitor of bioactive metabolites in WRS.
Highlights
Plants within the family, Brassicaceae, include some of the most popularly-consumed vegetables and are rich in several bioactive compounds capable of promoting human health, including carotenoids, vitamins, flavonoids and glucosinolates (GLs)
Non-Targeted Metabolome Analysis of White radish sprouts (WRS) Treated with Oligochitosan O-80
The sprouts exposed to 20 and 40 ppm of O-80 had a higher PC1 value compared to the others, while the WRS exposed to the lower dose clustered closely to the control treatment (0 ppm)
Summary
Brassicaceae, include some of the most popularly-consumed vegetables and are rich in several bioactive compounds capable of promoting human health, including carotenoids, vitamins, flavonoids and glucosinolates (GLs). White radish sprouts (WRS; Raphanus sativus, L.), a member of the Brassicaceae family, is studied in the present work as a source of health-promoting bioactive compounds, such as folic acid, dietary fiber, flavonoids, polyphenolics, vitamin A, vitamin C, and GLs [2,3]. 4-methylsulfinyl-3-butenyl isothiocyanate, called sulforaphene, the isothiocyanate derived from glucoraphenin, is predominant in radish sprouts.
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