Abstract
1. 1. Rat brain chromatin was fractionated by mild digestion with micrococcal nuclease into two fractions: “nuclease-sensitive” chromatin enriched in rapidly labelled RNA, total protein and nonhistone chromosomal proteins and “nuclease-resistant” chromatin with lower RNA and protein content. 2. 2. The “nuclease-sensitive” fraction comprised metabolically labile and stable non-histone proteins, while the “nuclease-resistant” fraction contained only metabolically stable proteins. 3. 3. The total and acid-soluble proteins in the “nuclease-sensitive” fraction were highly heterogeneous and contained several chromosomal proteins unique for this fraction.
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