Metabolic Basis for Injury to Plants from Combinations of O3 and SO2

Abstract

Pisum sativum L. cv Alsweet (garden pea) and Lycopersicon esculentum Mill. flacca (mutant tomato) were chosen to evaluate the metabolic basis for plant injury from combinations of O(3) + SO(2). The plants were exposed under conditions reported to specifically alter O(3) or SO(2) toxicity; light versus dark exposures, and treatment with the fungal metabolite fusicoccin (FC), the O(3) injury inhibitor N-[2-(2-oxo-1-imidazolidiny) ethyl]-N'-phenylurea (EDU), and the SO(2) injury stimulator diethyldithiocarbamate (DDTC). Plants were grown in controlled environment chambers and exposed to combinations of O(3) (0.05-0.2 microliters per liter) and SO(2) (0.1-0.3 microliters per liter) for 2 hours. Peas treated with FC had the same or greater injury (quantified by visual rating) with O(3) + SO(2) exposures compared to plants not treated with FC. For plants with open stomata in the dark as well as light, i.e. FC-treated peas and tomatoes, there was no change or an increase in foliar necrosis with O(3) + SO(2) exposures in the dark versus light. Peas treated with EDU had an almost complete absence of O(3) injury, no change in SO(2) injury, and moderate decreases in injury from combinations of O(3) + SO(2) compared to plants not treated with EDU. Tomatoes treated with DDTC showed the same or less injury compared to plants not treated with DDTC and exposed to O(3) or SO(2). The plant responses to the experimental treatments and O(3) + SO(2) resembled O(3) responses more than SO(2) responses. The evidence for O(3)-like responses are: no change or increase in injury in the light versus dark, and EDU-induced decreases in injury. Evidences for SO(2)-like responses are: incomplete protection from injury with EDU, and no change or increased injury to FC-treated versus untreated plants. Thus, a metabolic mechanism affected by both pollutants may be associated with the combination injury, e.g. effects the plasma membrane.