Metabolic activity of odontoblast-like cells irradiated with blue LED (455nm).

Abstract

Blue light emitting diodes (LEDs) are frequently used in dentistry for light activation of resin-based materials; however, their photobiostimulatory effects have not yet been fully investigated. This study aimed to investigate the effect of blue LED (455nm) on the metabolism of odontoblast-like cells MDPC-23. Energy doses of 2 and 4J/cm(2) were used at 20mW/cm(2) fixed power density. MDPC-23 cells were seeded at 10,000 cells/cm(2) density in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). After 12h, the culture medium was replaced with new DMEM supplemented with 0.5% of FBS, and the cells were incubated for further 12h. After that, single irradiation was performed to the culture, under selected parameters. Cell viability evaluations (Alamar Blue Assay, n = 12), number of viable cells (Trypan Blue Assay, n = 12), morphological analysis by scanning electron microscopy (SEM, n = 2), gene expression (n = 6) of alkaline phosphatase (Alp), collagen (Col-1a1), and dental matrix protein (Dmp-1) (quantitative polymerase chain reaction (qPCR)) were performed 72h after irradiation. Data were analyzed by Kruskal-Wallis, ANOVA, and Tukey tests (p < 0.05). Direct light application at 4J/cm(2) energy dose had no negative effects on cell viability, while irradiation with 2J/cm(2) reduced cell metabolism. None of doses affected the number of viable cells compared with the control group. The two energy doses downregulated the expression of Alp; however, expression of Col-1a1 and Dmp-1 had no alteration. Cells presented change in the cytoskeleton only when irradiated with 2J/cm(2). In conclusion, the blue LED (455nm) irradiation, under the evaluated parameters, had no biostimulatory effects on MDPC-23 cells.