Abstract
One approach available for the characterization of the eukaryotic genome involves the isolation of purified ribonucleic acid molecules and their use as probes into the fine structure of their complementary deoxyribonucleic acid templates. This method has been used in the analysis of genes coding for ribosomal RNA, 5 S RNA (Brown and Weber, 1968a,b; Birnstiel et al., 1968; Birnstiel et al., 1969), and transfer RNA (Clarkson and Birnstiel, this volume). It is only recently, however, that it has become possible to attempt a similar analysis of genes coding for proteins. The main problems have centered around the difficulty in obtaining from animal cells highly labeled, purified messenger RNAs that are known to code for a specific protein. Two mRNAs that have been isolated and purified, those for globin and silk fibroin, are discussed elsewhere in this volume. This paper concerns the isolation of purified messenger RNA for a specific histone...
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