Abstract
Recent studies have elucidated the role of several pro-inflammatory factors as mediators of inflammatory processes in the bovine endometrium. Only few studies, however, have analyzed samples collected from different regions of the uterus of the same animal. In this study, we tested the hypothesis that on a molecular level, clinical endometritis is characterized by inflammatory responses spread over the entire endometrium. Furthermore, we assume that subclinical endometritis is described by an inflammation of local regions of the uterus. Therefore, the objective of this study was to assess the mRNA expression of uterus-associated pro-inflammatory factors at five pre-defined endometrial sites, i.e., corpus uteri, left horn base, left horn tip, right horn base, and right horn tip, in cows with clinical and subclinical endometritis and in healthy controls. We analyzed the mRNA expression of interleukin 1 alpha, interleukin 1 beta, C-X-C motif chemokine ligand 8, prostaglandin-endoperoxide synthase 2, protein tyrosine phosphatase receptor type C, carcinoembryonic antigen related cell adhesion molecule 1, and mucin 4 and 16. Based on vaginoscopy and endometrial cytology (≥ 5% polymorphonuclear neutrophils) between 28 to 34 days in milk, 18 Simmental cows were categorized in clinical endometritis group (n = 7), subclinical endometritis group (n = 4), and healthy group (n = 7). In general, the analyses revealed a great variation of mRNA expression between sites and animals. Differences were found between different uterine health statuses, but the variation between the sampling sites within the groups was not significant (P > 0.05). This indicates that inflammatory processes at the end of the postpartum period can be regarded as multi-focal or spread throughout the uterus independent from the uterine health status.
Highlights
Postpartum uterine disorders, such as metritis, clinical endometritis (CE), and subclinical endometritis (SE), have negative short- and long-term impact on bovine fertility [1,2,3]
We tested the hypothesis that on a molecular level, clinical endometritis is characterized by inflammatory responses spread over the entire endometrium
The mRNA expression of certain cytokines and glycoproteins are dependent on the health status of the bovine endometrium, the stage of the estrus cycle and anatomical sections of the uterus [15,16,17]
Summary
Postpartum (pp) uterine disorders, such as metritis, clinical endometritis (CE), and subclinical endometritis (SE), have negative short- and long-term impact on bovine fertility [1,2,3]. The cytobrush (CB) technique was reported as a reliable and accurate method for endometrial sampling and the diagnosis of SE by determining the proportion of PMN in the smears [9,10,11]. It has been discussed whether a single sample is representative for the entire endometrium and affects the accuracy of the diagnosis of SE [reviewed by [12, 13]]. Repeated sampling of the same cows showed no or only few intra-cow variations of the mRNA expression of selected factors [16, 17]
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