Mesoscopic Multimodal Imaging Provides New Insight to Tumor Tissue Evaluation: An Example of Macrophage Imaging of Hepatic Tumor using Organosilica Nanoparticles

Michihiro Nakamura,Masafumi Harada,Koichiro Hayashi,Keisuke Izumi,Yoshihiro Tsuruo,Toshinobu Yogo,Hitoshi Kubo

doi:10.1038/s41598-017-04043-7

Michihiro Nakamura, Masafumi Harada + Show 5 more

Open Access

https://doi.org/10.1038/s41598-017-04043-7

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Abstract

Multimodal imaging using novel multifunctional nanoparticles provides new approach to biomedical field. Thiol-organosilica nanoparticles containing iron oxide magnetic nanoparticles (MNPs) and rhodamine B (thiol OS-MNP/Rho) were applied to multimodal imaging of hepatic tumor of Long−Evans Cinnamon (LEC) rat. The magnetic resonance imaging (MRI) of LEC rats revealed tumors in the liver clearly and semi-quantitatively due to a labeling of macrophages in liver. The fluorescent imaging (FI) showed abnormal fluorescent patterns of the liver at the mesoscopic level that was between macroscopic and microscopic level. We performed correlation analysis between optical imaging including FI and MRI. We found that the labeled macrophages located specific area in the tumor tissue and influenced the tumor size on MRI. In addition histological observation showed the labeled macrophages related specific tissue in the pathological region. We demonstrated a new approach to evaluate tumor tissue at the macroscopic and microscopic level as well as mesoscopic level using multimodal imaging.

Highlights

Various types of multimodal imaging nanoparticles (NPs) have been investigated and applied for magnetic resonance imaging (MRI) and fluorescent imaging (FI) of hepatic tumor[11,12,13]
We report the first usefulness of multimodal imaging of pathological liver in Long−Evans Cinnamon (LEC) rats with a fusion of optical imaging including FI and T1- and T2-weighted MRI using thiol-OS-magnetic nanoparticles (MNPs)/Rho
The absorbance spectra of thiol-OS-MNP/Rho showed a peak at 563 nm, while rhodamine B appears at 554 nm (Fig. 1Bc)

Summary

Provides New Insight to Tumor

Received: 17 February 2017 Accepted: 8 May 2017 Published: xx xx xxxx of Macrophage Imaging of Hepatic Tumor using Organosilica. Thiol-organosilica nanoparticles containing iron oxide magnetic nanoparticles (MNPs) and rhodamine B (thiol OS-MNP/Rho) were applied to multimodal imaging of hepatic tumor of Long−Evans Cinnamon (LEC) rat. We demonstrated a new approach to evaluate tumor tissue at the macroscopic and microscopic level as well as mesoscopic level using multimodal imaging. Various types of multimodal imaging nanoparticles (NPs) have been investigated and applied for MRI and FI of hepatic tumor[11,12,13]. Multimodal imaging NPs containing magnetic nanoparticles (MNPs) and fluorescent nanoparticles (FNPs) can be applied to T2-weighted MRI and FI. Importance of multimodal “macrophage imaging” to evaluate hepatic tumor tissue has not been reported very well. We demonstrated that “differential” multimodal imaging of hepatic tumor between optical imaging including FI and MRI was very important to evaluate and understand tumor tissue. We discuss a new insight of hepatic tumor tissue using multimodal imaging

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