Mesoporous Calcium-Silicate Nanoparticles Loaded with Low-Dose Triton-100+Ag+ to Achieve Both Enhanced Antibacterial Properties and Low Cytotoxicity for Dentin Disinfection of Human Teeth.

Mengting Duan,Wei Fan,Bing Fan

doi:10.3390/pharmaceutics13091518

Abstract

Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag+) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacterial properties and low cytotoxicity for dentin disinfection. The physicochemical property, biocompatibility, infiltration ability into dentinal tubules, anti-bacterial ability against both planktonic Enterococcus faecalis (E. faecalis) and its biofilm on dentin, effects on dentin microhardness and in vitro mineralization property were systematically investigated. Results confirmed that the MCSNs and M-AgTX nanoparticles showed typical morphology of mesoporous materials and exhibited sustained release of chemicals with an alkaline pH value over time. M-AgTX also exhibited excellent biocompatibility on MC3T3-E1 cells and could eliminate 100% planktonic E. faecalis after 48-h treatment. On dentin slices, it could enter dentinal tubules by ultrasonic activation and inhibit the growth of E. faecalis on dentin. M-AgTX could completely inactive 28-day E. faecalis biofilm. TEM confirmed the destruction of cell membrane integrity and Ag+ infiltration into bacteria by M-AgTX. Besides, dentin slices medicated with M-AgTX nanoparticles displayed an increased microhardness. After being immersed in SBF for 7 days, apatite crystals could be observed on the surface of the material tablets. M-AgTX could be developed into a new multifunctional intra-canal medication or bone defect filling material for infected bone defects due to its sustained release profile, low cytotoxicity, infiltration ability, enhanced anti-bacterial and mineralization features.

Highlights

Enterococcus faecalis (E. faecalis) is a Gram-positive facultative anaerobic bacterium reported to be resistant to many anti-bacterial medications or agents, that can cause refractory infection and reinfection of the root canal system of human teeth [1,2]
FE-SEM and TEM revealed that Mesoporous calcium-silicate nanoparticles (MCSNs) and M-AgTX possessed spherical morphology with less than 100 nm diameter (Figure 1A,B,D,E)
TEM images showed that the mesoporous structures had well-ordered nanopores and channel structures, and numerous black dots were trapped within the M-AgTX nanoparticles (Figure 1B,E)

Summary

Introduction

Enterococcus faecalis (E. faecalis) is a Gram-positive facultative anaerobic bacterium reported to be resistant to many anti-bacterial medications or agents, that can cause refractory infection and reinfection of the root canal system of human teeth [1,2]. The search for more effective antibacterial agents against E. faecalis has always been a research focus of root canal disinfection studies [4]. Calcium hydroxide (Ca(OH)2 ) is the most popular intracanal disinfection medicament so far due to its high alkaline pH due to the hydroxyl ions it releases [5]. It is unable to eliminate E. faecalis as E. faecalis shows strong resistance to alkalinity, and the prolonged treatment with Ca(OH) could result in reduced flexural strength and fracture resistance of dentin, increasing the risk of tooth fractures [6,7,8].

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