Mesophilic condition favors simultaneous partial nitrification and denitrification (SPND) and anammox for carbon and nitrogen removal from anaerobic digestate food waste effluent

Abstract

Three simultaneous partial nitrification and denitrification (SPND) bioreactors were established on ambient (30 °C), mesophilic (40 °C) and thermophilic condition (50 °C) at high dissolved oxygen levels (2–7 mg L−1) to remove nitrogen and carbon from anaerobic digestate food waste effluent (ADFE). The bioreactor performed best under mesophilic condition, with TN and COD removal efficiency of 96.3 ± 0.1% and 91.7 ± 0.1%, respectively. Free ammonia (FA) and free nitrous acid (FNA) alternately ensured selective inhibition of nitrite-oxidizing bacteria (NOB) in long-term operation of SPND systems. Candidatus Brocadia, known as anammox bacteria, was observed unexpectedly in the bioreactors. The analysis of microbial community and metabolic pathways revealed that mesophilic strategy stimulated SPND and anammox process. Mesophilic condition helped autotropic microbes resist the competitive pressure from heterotrophic bacteria, improving the balance between nitrifiers, anammox bacteria and other co-existing heterotrophs. Overall, this study offers new insights into the linkage among temperature, pollutant removals (carbon and nitrogen) and metabolic potential in the SPND bioreactors.