Abstract
Clostridia were isolated from marine sediments collected in Puget Sound in the State of Washington. Sampling stations varied in depth from 10 to 240 m and sediment types varied from sand and gravel to reduced mud. Counts of bacteria growing both aerobically and anaerobically at 30 °C were made. Aerobic and anaerobic counts were 1.21 to 16.9 × 104 and 0.73 to 23.5 × 104 cells/ml of sediment–water slurry respectively. About 30% of the bacteria growing anaerobically were obligate anaerobes and identified as Clostridia. The identified Clostridia fell into 12 groups. The first three groups, containing Clostridium perfringens, C. bifermentans, and C. novyi, made up 76% of the isolates.The results show a variety of species of mesophilic Clostridia in marine sediments with C. perfringens isolated in the highest numbers.
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