Mesocosm-based simulations to optimize a bioremediation strategy for the effective restoration of wildfire-impacted soils contaminated with high-molecular-weight hydrocarbons.

Abstract

We obtained four microbial isolates from soil exposed to forest fire and evaluated their potential bioremediation activity when combined with a biosurfactant-producing bacterial strain for the decontamination of wildfire-impacted soil polluted with high-molecular-weight (HMW) hydrocarbons. We established mesocosm trials to compare three bioremediation strategies: natural attenuation, bioaugmentation and biostimulation. Chemical analysis, culture-dependent and culture-independent methods were used to evaluate the bioremediation efficiency and speciation of the microbial cenoses based on these approaches. After treatment for 90days, bioaugmentation removed 75·2-75·9% of the HMW hydrocarbons, biostimulation removed 63·2-69·5% and natural attenuation removed ~22·5%. Hydrocarbon degradation was significantly enhanced in the mesocosm supplemented with the biosurfactant-producing bacterial strain after 20 and 50days of treatment compared to the other bioremediation strategies. We found that the bioaugmentation approach was more effective than biostimulation and natural attenuation for the removal of HMW hydrocarbons from fire-impacted soil. Our study showed that micro-organisms from wildfire-impacted soil show significant potential for bioremediation, and that biosurfactant-producing bacterial strains can be combined with them as part of an effective bioremediation strategy.