Abstract
Background / AimsNew therapeutic targets need to be identified in prostate cancer which remains the second cause of cancer related death in US men. Some studies suggest that MSCs may be candidates for cancer transformation in a gastric cancer model. We aim at understanding whether MSCs have a role in prostate tumorigenesis.MethodsCells were obtained and expanded in vitro from 7 CaPs specimens, after fluorescence‐activated cell sorting or after multiple steps of cell adhesion, and eventually characterized by immunocytochemistry and FACS then injected into a xenochimeric mouse model.ResultsMSCs markers (CD9, CD13, CD44, CD73 and CD90) were expressed by 95% to 100% of the cells. Eight weeks after in vivo injection under the renal capsule of immunodeficient mice, in presence of fetal rat UGM, cells from 3 specimens (3/3) generated xenografts, while fetal rat UGM alone and cells alone did not grow. Further analysis of the grafts show the presence of glandular structures with prostate intraepithelial neoplasia features and some glands were of human origin.ConclusionsOur results suggest that MSCs grown from prostate tumors, under appropriate stimulation, are able to generate glandular structures with preneoplastic features. Further characterization of the respective origin of the glands, vessels, and stroma, is mandatory to further elucidate the potential of these MSCs.
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