Abstract

Background: The mechanisms underlying peripheral disorders during systemic lupus erythematosus (SLE) were found to be shared with tolerance disorders and mediated by T-regulator (T-reg) cells. Mesenchymal stem cells (MSCs) may inhibit T-cell subset differentiation and induce the T-reg cell phenotype. However, the capacity of MSCs to promote functional T-reg cells in SLE patients remains unclear.
 Objectives: This study aimed to analyze the capacity of MSCs to induce the production of functional CD4+ CD25+ Foxp3+ T-reg cells, in vitro, under co-culture conditions with human SLE cells.
 Methods: This study used a pre- and post-test control group design. Peripheral blood mononuclear cells (PBMCs) were extracted from SLE patients at the Kariadi Hospital, and MSCs were derived from human umbilical cords (hUCs) The PBMC control group was treated with standard medium, and the treatment group was co-cultured with hUC-MSCs. After 24 hours of co-culture incubation, T-reg cells were removed from the PBMC pool, using magnetic-activated cell sorting (MACS), and the population was assessed using the trypan blue exclusion assay.
 Results: A significant increase in the population of T-reg cells was observed (P < 0.001) after 24 hours of co-culture incubation with hUC-MSCs.
 Conclusion: This study concluded that MSCs have the capacity to enhance the T-reg population in human SLE PBMCs.
 Bangladesh Journal of Medical Science Vol.19(4) 2020 p.743-748

Highlights

  • Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease, in which the body’s immune system produces excessive antibodies or other soluble molecules that trigger excessive inflammatory responses[1,2]

  • This research used peripheral blood mononuclear cells (PBMCs), which were extracted from systemic lupus erythematosus (SLE) patients at the Kariadi Hospital after obtaining informed consent, and Mesenchymal stem cells (MSCs) derived from human umbilical cords

  • The control group comprised Peripheral blood mononuclear cells (PBMCs) treated with standard medium, and the treatment group comprised PBMCs cocultured with human umbilical cords (hUCs)-MSCs

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Summary

Introduction

Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease, in which the body’s immune system produces excessive antibodies or other soluble molecules that trigger excessive inflammatory responses[1,2]. SLE patients continue to be at increased risk for premature mortality, according to a cohort study (1999-2014), indicating that mortality has not significantly improved among SLE patients, among young adults[3]. The capacity of MSCs to promote functional T-reg cells in SLE patients remains unclear. Objectives: This study aimed to analyze the capacity of MSCs to induce the production of functional CD4+ CD25+ Foxp3+ T-reg cells, in vitro, under co-culture conditions with human SLE cells.

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