Mesenchymal stem cell conditioned medium azacytidine, panobinostat and GSK126 alleviate TGF-β-induced EMT in lung cancer

Huihui Shen,Dongying Zhang,Hua Liu

doi:10.1590/fst.53021

Abstract

This study aimed to investigate the effects of mesenchymal stem cells (MSCs) conditioned medium, azacytidine, panobinostat, and GSK126 on TGF-β-induced EMT in lung cancer. MSC were cultured in DMEM medium to make MSC conditioned medium. Our results showed that MSC conditioned medium, azacytidine, panobinostat and GSK126 reduced cell viability, invasion, expressions of N-cad, vimentin and α-SMA and increased E-cad expression in cells treated with TGF-β. MSC conditioned medium alleviated TGF-β-induced increases in cell viability and decrease in E-cad expression. In conclusion, MSC conditioned medium, azacytidine, panobinostat, and GSK126 could alleviate TGF-β-induced EMT in lung cancer.

Highlights

Lung cancer is the most common primary malignant tumor with high morbidity, rapid growth, high mortality and poor prognosis (Torre et al, 2016)
Practical Application: We investigated the effects of mesenchymal stem cells (MSCs) conditioned medium, azacytidine, panobinostat, and GSK126 on TGF-β-induced Epithelial-mesenchymal transition (EMT) in lung cancer
In order to study the influences of MSC conditioned medium and three inhibitors azacytidine, panobinostat and GSK126 in

Summary

Introduction

Lung cancer is the most common primary malignant tumor with high morbidity, rapid growth, high mortality and poor prognosis (Torre et al, 2016). Lung cancer has become a serious global public health problem (Kesika et al, 2020; Wang et al, 2021; Zhao & Zhan, 2020). A study found that the most cause of death in lung cancer patients was the invasion and metastasis of tumor cells (Chen et al, 2008). How to find lung cancer as soon as possible, and effectively control the invasion and metastasis of cells become an urgent problem to be solved in the treatment of lung cancer (Vasconcelos et al, 2019; Rafiq et al, 2020; Satelli & Li, 2011)

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