Abstract
AbstractA merocyanine dye‐based fluorescent probe have been synthesised for selective and sensitive detection of hypochlorous acid. The merocyanine dye 1 can be synthesized by simple condensation reaction of 1, 1, 2, 3‐tetramethyl‐1H‐benzo[e]indol‐3‐ium iodide with indole‐3‐carboxaldehyde. The reaction between hypochlorous acid and dye 1 is linear in the hypochlorous acid concentration range of 0 to 100 μM with a detection limit of 42 nM by fluorescent spectrometry method. The intramolecular charge transfer based fluorescent probe dye 1 displayed a considerable fluorescent spectrum at 425 nm enhancement and the color changes from red to colorless by naked eye detection in the addition of hypochlorous acid. Further supported by the 1H NMR titrations reveal that the reactive oxygen species in dye 1 bind to the indole‐NH‐unit. The chemosensor also showed excellent performance when used in the “test strip” method.
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