Abstract

Meristematic activity in shoot apices of NCTG-22, a short-day (SD) sensitive cultivar of Nicotiana tabacum, was monitored throughout the 17-d period of transition from vegetative to reproductive growth The analysis included changes in (1) size of the apical meristem, (2) rate of leaf initiation, (3) amount of nuclear DNA, and (4) percentage of cells in division. Plants grown in controlled environment chambers set at 26/22 C day/night temperatures were exposed either to an inductive short-day photoperiod or a noninductive long-day (LD) photoperiod after a 6-wk juvenile period. The first morphological change associated with floral onset was an acceleration in the rate of leaf initiation following three inductive cycles, which continued and resulted in the eventual production of three more leaves by SD plants than by vegetative LD plants Apices were identical in size until seven inductive cycles were completed, after which SD apices began to enlarge, finally becoming more than twice as wide as LD apices and ...

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