MePOD12 participates the regulation to postharvest physiological deterioration by ROS scavenging and lignin accumulation in cassava tuberous roots

Abstract

Cassava tuberous root, as a staple food crop and industrial raw material worldwide, is highly sensitive to postharvest physiological deterioration (PPD) caused by wound. However, the molecular mechanism underlying PPD tolerance remains elusive in cassava. The reactive oxygen species (ROS) scavenging system is activated at the preliminary stage of PPD and peroxidase (POD) can effectively eliminate ROS. The expression level of MePOD12 and POD enzymatic activity in cassava tuberous roots was considerably up-regulated with PPD occurrence. Yeast two-hybrid and luciferase reporter assay showed that MePOD12 physically interacted with lignin-related cinnamyl alcohol dehydrogenase 15 (MeCAD15) in vitro and in vivo. The MePOD12-silenced tuberous roots decayed more than control, especially stored at room temperature for 3 and 5 days after harvesting. The total antioxidant capacity, activities of POD, SOD, CAT, CAD and lignin content were lower than those of control. Particularly, after harvest for 3 days, the accumulation of hydrogen peroxide and superoxide anion substantially increased compared to control. In addition, a proposed model was generated to elaborate the response mechanism of MePOD12 participating in PPD regulation via ROS elimination and lignin accumulation. It provides a new clue for breeding cassava PPD-tolerant varieties.