Meniscus-Assisted High-Efficiency Magnetic Collection and Separation for EWOD Droplet Microfluidics

Abstract

This paper describes a technique to increase the efficiency of magnetic concentration on an electrowetting-on-dielectric (EWOD)-based droplet (digital) microfluidic platform operated in air, i.e., on dry surface. Key differences in the force scenario for droplet microfluidics vis-a-vis the conventional continuous microfluidic systems are identified to explain the rationale behind the proposed idea. In particular, the weakness of the magnetic force relative to the bead-substrate adhesion and the liquid-air interfacial tension is highlighted, and a new technique to achieve high-efficiency magnetic collection with the assistance of the interfacial force is proposed. An improvement in collection efficiency (e.g., from ~ 73% to ~ 99%) is observed with the new technique of ldquomeniscus-assisted magnetic bead collectionrdquo. In addition, isolation of the magnetic species from a mixed sample of magnetic and nonmagnetic beads is demonstrated. Comparison with other related reports is also presented.