Abstract
ABSTRACTMeniscal damage is, despite its major role in knee osteoarthritis (OA), often neglected in OA animal models. We evaluated structural meniscal degeneration during the course of OA in the murine collagenase‐induced OA (CIOA) model. To investigate this, OA was induced in the knee joints of 33 male C57BL/6 mice by an intra‐articular injection of 10U collagenase. The mice were sacrificed after 1, 3, 7, 14, 28, and 56 days, and the knees were harvested and processed for histological analysis. As control, six knees were obtained from 16‐week‐old mice in which no OA was induced. Meniscal damage, meniscal extrusion, and articular cartilage damage were evaluated on thionin‐stained sections. Associations between parameters of interest were evaluated with Spearman rho correlation tests. When compared to non‐OA knees, meniscal extrusion was visible from day 1 onwards and meniscal degeneration had a tendency to increase over time. The meniscus damage appeared around the same time as articular cartilage damage (day 14–28) and was statistically significantly more pronounced anterior than posterior, and no differences were seen between medial and lateral menisci. Meniscus and articular cartilage damage were moderately associated in the CIOA knees (ρ = 0.57; 95%CI [0.23–0.78]). Our findings suggest that the CIOA model is a valuable model to study the role of meniscal damage during OA progression and can support the development of future preventative treatment strategies. © 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:2416–2420, 2018.
Highlights
Meniscal degradation is, in spite of its critical role in knee osteoarthritis (OA), often neglected in OA animal models
Meniscus and articular cartilage damage were moderately associated (r 1⁄4 0.57; 95% confidence interval (95%CI) [0.23–0.78]; Fig. 2C) in the collagenase-induced OA (CIOA) knees and the meniscus damage appeared around the same time as articular cartilage damage
To conclude, several studies have shown a correlation between extrusion of the meniscal body and knee OA8,9 and meniscal extrusion is known to be independently related to cartilage loss.[8,9,10,22]
Summary
In spite of its critical role in knee osteoarthritis (OA), often neglected in OA animal models. A systemic evaluation method for degeneration of the meniscus in experimental OA was established by Kwok et al.[16] In the study, the authors reported insights on the structural changes of the menisci during aging and OA and have shown that in the DMM model, meniscal damage and articular cartilage damage develop synchronously from day 14 onwards. The aim of this study was to evaluate meniscal damage during the course of experimental knee OA in the CIOA mouse model, immediately after OA onset
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