Abstract
1. 1. Menadione is known to decrease the mixed function oxidase mediated metabolism of a number of substrates in microsomal systems. 2. 2. The present study examines the effect of menadione on benzo(a)pyrene metabolism in whole cells, microsomes and a semi-purified reconstituted mixed function oxidase system. 3. 3. Menadione has a high affinity for the NADPH dependent cytochrome - P450 reductase and acts as a competitive inhibitor of cytochrome P-450 reductase in the metabolism of benzo(a)pyrene. 4. 4. This is the mechanism of inhibition of aryl hydrocarbon hydroxylase by menadione in reconstituted systems. 5. 5. In a whole cell system and at low concentrations of menadione, depletion of reduced pyridine nucleotides is the initial inhibitory event.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.