MEMS or microchip based capillary electrophoresis tool for analysis of Pathogenesis-related (PRs) proteins in Eruca sativa (Miller) cultivars against Fusarium oxysporum

Abstract

Plants provide a defense mechanism against various pathogens or biotic stress through various strategies. Plants respond to fungal invasion by activating defense responses associated with accumulation of protein content profile and pathogenesis-related proteins (PRs), which prevent pathogen infection. The study was focus on the role of the total protein profile and analysis of PRs proteins through micro electromechanical systems (MEMS) based capillary electrophoresis (CE) tool in taramira (Eruca sativa Mill.) cultivars, resistant (RTM-1212) and susceptible (RTM-2002 and RTM-314) to Fusarium oxysporum was investigated in 15 and 25 days old plants. Various revolutionary ideas have sprung up in the field of biomedical applications due to rapid development of MEMS technology. A chemical sensing device in which a biologically derived recognition analyte is coupled to a transducer to allow the quantitative development of biochemical parameter is called a biosensor. CE is an important separation and detection technique which has emerged as a powerful technique for this purpose. It is used for the separation and detection of a wide range of analytes ranging from heavy metal ions to detection of phenolic acid in extracts from plant and samples. Total protein content profile and PR-protein were higher in pathogen inoculated plants as compared to control plants with an increase till 168 h (long time period) and 10 h (short time period). The obtained results give important information concerning the plant-pathogen interactions, in the defense response for E. sativa improvement programs seeking the adaptation to diverse range of fungal attack along with adverse environmental factors.