Abstract
The absorption of phosphate ion by Hymenolepis diminuta involved diffusion at all substrate concentrations tested. Mediated transport predominated at lower substrate concentrations and it exhibited saturation kinetics with Vmax, and Km values of 0.056 µmol ethanol-extractable inorganic phosphate/g ethanol extracted dry wt/2 min and 1.19mM, respectively, obtained from the Lineweaver-Burk transformation. The Vmax and Km values determined by the Hanes-Woolf phot were 0.136 µmol ethanol-extractable inorganic phosphate/g ethanol extracted dry wt/2 min and 3.64 mM, respectively. Absorption was influenced by changes in pH, temperature, and sodium ion concentration. Stable inorganic phosphate inhibited transport competitively. Various organic phosphates and common metabolic poisons introduced had no effect on transport. However, transport was inhibited by molybdate. From these data, it can be deduced that a separate transport system exists for phosphorus in H. diminuta.
Submitted Version (
Free)
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
