Membrane retrieval following exocytosis in isolated neurosecretory nerve endings

Abstract

In the neurosecretory nerve endings of the neurohypophysis depolarization-induced exocytosis is followed by endocytosis of vacuole-like structures with diameter similar to that of neurosecretory granules. However, it remains unknown whether the membrane of the endocytotic vacuoles is comprised primarily of retrieved secretory granule membrane, plasma membrane or of a mixture of the granule and plasma membrane. In the present paper membrane retrieval following depolarization-induced exocytosis has been studied in isolated neurosecretory nerve endings from the rat neurohypophysis. The origin of the retrieved membrane was assessed by pre-labeling the plasma membrane with an antibody against neural cell adhesion molecule, a plasma membrane specific protein. Horseradish peroxidase was used as an index of fluid endocytosis and secretion of vasopressin was measured by radioimmunoassay. Following potassium-induced depolarization, endocytotic vacuoles showed labeling with the fluid phase marker horseradish peroxidase but never showed significant neural cell adhesion molecule labeling. The time-course of endocytosis following closely that of exocytosis as endocytotic vacuoles labeled with horseradish peroxidase were only observed when the fluid phase marker was present in the extracellular medium during the period of evoked exocytosis. Our results are consistent with a model in which in neurosecretory nerve endings, after transient exocytotic fusion of the granule membrane with the plasma membrane, the granule membrane is rapidly and selectively retrieved into the nerve endings in the form of vacuoles similar in size to that of the neurosecretory granules.