Membrane proteins: Isolation and characterization: edited by A. Azzi, L. Masotti and A. Vecli. Springer—Verlag, Berlin, 1986, 195 p., DM 38

Abstract

I. Membrane Proteins: Function, Purification, and Characterization.- Purification of Cytochrome c Reductase and Oxidase by Affinity Chromatography.- Molecular Weight Estimation of Membrane Proteins: Gel Permeation Chromatography and Sucrose Gradient Centrifugation.- The Problem of Light Scattering in the Absorbance and Fluorescence Studies of Proteins in Membranes.- Reproducible Preparation of Phosphatidylserine Vesicles for Fluorescence Studies of Protein Incorporation into Lipids.- Purification and Reconstitution of the Proton Translocating Membrane Bound Inorganic Pyrophosphatase from Rhodospirillum rubrum.- The Function of Transmembrane Channels: Ion Transport Studies by 23Na NMR.- Membrane Signal Transduction via Protein Kinase C.- Extraction, Partial Purification, and Reconstitution of a Mixture of Carriers from the Inner Mitochondrial Membrane.- II. Lipid-Protein Interaction.- The Effect of Polypeptides on the Fluidity of Lipid Bilayers.- Polypeptide-Lipid Interactions as Studied by 13C NMR.- Conformational Changes in Polypeptides and Proteins Brought About by Interactions with Lipids.- III. Protein Modification.- Two Examples of Selective Fluorescent Labeling of SH-Groups with Eosin-5-Maleimide: The ADP/ATP Translocator and the Cytochrome c Oxidase Subunit III of Bovine Heart Mitochondria.- Hydrophobic Photolabeling with 125I-TID of Red Blood Cell Membranes.- Use of Fluorescent Probes of the Adenine Nucleotide Carrier for Binding Studies and Analysis of Conformational Changes.- Spin Labeling of Membranes and Membrane Proteins.- IV. Protein Reconstitution.- Functional Reconstitution of the Mitochondrial Cytochrome b-c1 Complex: Effect of Cholesterol.- Changes of the Membrane Surface Potential Measured by Amphiphilic Fluorescent and ESR Probes.- Reconstitution of Cytochrome c Oxidase.- Measurement of Pyruvate Transport in Mitochondria.