Membrane protein synthesis after infection of Escherichia coli B with phage T4: The rIIB protein

Abstract

Inner and outer cell membrane proteins synthesized after infection of Escherichia coli B with wild-type phage T4, rIIA and B mutants and a spackle mutant were analyzed by SDS-polyacrylamide gel electrophoresis. At least three new proteins of approximate molecular weights 22,000, 29,000, and 37,000 daltons are made after infection with wild-type phage. The protein of molecular weight 37,000 is not present in membranes of cells infected with an rIIB deletion mutant. This protein is the major protein made from 5 to 15 minutes after infection with the wild type. In addition, the major host protein of the outer envelope is not made during wild-type phage infection.