Membrane potential, action potential and activation potential of eggs of the sea urchin, Lytechinus variegatus

Abstract

Unfertilized Lytechinus variegatus eggs in sea water in their normal physiological state have membrane potentials that approximate −70 to −80 mV. This conclusion is based on microelectrode measurements and on computation from the Na + and K + fluxes. The −8 to −15 mV values for the membrane potential previously reported and which are generally measured are the consequence of depolarization by impalement. The activation potential in inseminated eggs with an initial membrane potential more negative than −60 mV is a compound event involving sperm-induced as well as voltage dependent conductance changes. The sperm-induced mechanism is a two-phase conductance increase which involves both Na + and Ca 2+ during the first phase, and Na + alone during the second phase. In addition, the sperm-induced depolarization at the beginning of the first phase activates a voltage dependent Ca 2+-conductance mechanism resulting in generation of an action potential.