Abstract
Partitioning of hydrophobic cations, tetraphenylphosphonium (TPP+) and triphenylphosphonium homologues ((Phe)3−P+−(CH2)nCH3, n = 0−6), into lipid bilayers was studied by chromatography on immobilized small and large unilamellar liposomes (SUVs with mean diam of 30 nm and LUVs with 100 nm). The liposomes were immobilized stably in chromatographic gel beads by avidin−biotin (Yang et al. J. Chromatogr. B. 1998, 707, 131). By immobilized liposome chromatography (ILC), membrane partition coefficients (KLM) of the cations were measured. The KLM values were coincident with molar partition coefficient, Km, which was determined for some of cations by an ultrafiltration method. Temperature dependence of KLM gave the thermodynamic quantities of membrane partitioning, ΔH° (enthalpy change) and ΔS° (entropy change). The same quantities were also measured with isothermal titration calorimetry (ITC), and the values of both methods were similar to each other. This confirms the usefulness of ILC on the basis of its simpli...
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