Abstract
Membrane interactions and cellular uptake of an amphipathic cell-penetrating peptide as a delivery system for miRNA
Highlights
Cell-penetrating peptides constitute a promising strategy for the intracellular delivery of therapeutic molecules, such as nucleic acids
The non-covalent approach based on the amphipathic N-TER peptide has shown to be successful in the intracellular delivery of miRNA-27a by inducing its antiadipogenic effect in 3T3-L1 cells
The peptide increases the membrane fluidity and improves its own cellular uptake (Gerbal-Chaolin et al, 2007). This is in accordance with the images obtained using CLSM, where there is a significant difference in the cytoskeleton of cells treated either with the peptide alone, or complexed with Fluo-non-targeting control (NTC), compared to the untreated cells
Summary
Cell-penetrating peptides constitute a promising strategy for the intracellular delivery of therapeutic molecules, such as nucleic acids. The non-covalent approach based on the amphipathic N-TER peptide has shown to be successful in the intracellular delivery of miRNA-27a by inducing its antiadipogenic effect in 3T3-L1 cells. Information regarding the events taking place at the cellular interface is lacking. Information on the uptake process often comes as confusing and contradictive. The main focus of our study is to examine how the peptide interacts with the cell membrane, is this interaction fatal for the cells, or is it just the first step towards the peptide’s internalization into the cells?
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