Abstract
A simplified model system is introduced to elucidate the significance of the ω-functionalised fatty acid moiety of muraymycin nucleoside antibiotics for membrane interaction and penetration.
Highlights
Bacterial strains with resistances towards established antibiotics continue to emerge
Using the copper-catalysed version of the Huisgen alkyne–azide cycloaddition (“click”-chemistry),[16,17,18] this should furnish AlexaFluor 488 (AF488) LSC conjugate 11. It can be tested if the presence of the LSC unit makes the AF488 moiety membrane-permeable, i.e. if fluorescence can be detected within lipid vesicles upon treatment with 11 (Fig. 2A)
We were able to demonstrate that the ω-functionalised fatty acid moiety found in some muraymycin nucleoside antibiotics of the A-series can mediate membrane accumulation and even membrane penetration of a fluorescent dye
Summary
Bacterial strains with resistances towards established antibiotics continue to emerge. Membrane partitioning assay The influence of AlexaFluor 488 LSC conjugate 11 on lipid membranes was investigated by means of fluorescence microscopy based on giant unilamellar vesicles (GUVs).
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